{"id":523,"date":"2021-12-23T22:37:43","date_gmt":"2021-12-23T22:37:43","guid":{"rendered":"https:\/\/startersites.io\/blocksy\/garderobe\/?post_type=product&p=523"},"modified":"2026-06-08T17:08:48","modified_gmt":"2026-06-08T17:08:48","slug":"color-sneakers","status":"publish","type":"product","link":"https:\/\/bullziye.com\/?product=color-sneakers","title":{"rendered":"FluoSpheres\u2122 Polystyrene Microspheres, 1.0 \u03bcm, blue-green fluorescent (430\/465), for tracer studies"},"content":{"rendered":"
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes\u2122 FluoSpheres\u2122 beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport\u00a0in vivo<\/i>, etc., we offer more concentrated, 0.04 \u03bcm diameter FluoSpheres\u2122 microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres\u2122 products. The 0.04 \u03bcm particles are small enough to be microinjected or to be taken up by phagocytosis.

We also offer 1.0 \u03bcm FluoSpheres\u2122 microsphere preparations that contain a much higher dye content than our other FluoSpheres\u2122 products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.

In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres\u2122 beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.

FluoSpheres\u2122 Microsphere Specifications:<\/b>

\u2022\u00a0Label (Ex\/Em): Blue-green fluorescent (430\/465)<\/b>
\u2022\u00a0Nominal bead diameter: 1.0 \u03bcm<\/b>
\u2022\u00a0Coupling surface: Carboxylate<\/b>
\u2022\u00a0Solids: 2%<\/b>

Characteristics of the Carboxylate Coupling Surface<\/b>
Carboxylate-modified FluoSpheres\u2122 beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres\u2122 Fluorescent Microspheres<\/b>
In addition to\u00a0microspheres for tracing<\/a>, investigate our\u00a0complete offering of fluorescent microspheres products<\/a>. Among those products you\u2019ll find beads with these variations:
\u2022 Ten fluorescent colors
\u2022 Ten nominal bead diameters: 0.02 \u03bcm, 0.04 \u03bcm, 0.1 \u03bcm, 0.2 \u03bcm, 0.5 \u03bcm, 1.0 \u03bcm, 2.0 \u03bcm, 4.0 \u03bcm, 10.0 \u03bcm, and 15.0 \u03bcm
\u2022 Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
\u2022 Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres<\/b>
We also offer hundreds of choices for\u00a0UltraClean\u2122 surfactant-free microspheres<\/a>\u00a0for research and commercial applications.

We\u2019ll Make a Custom Microsphere Product for You<\/b>
We will prepare\u00a0custom orders<\/a>\u00a0upon request. For example, FluoSpheres\u2122 beads can be prepared with intensities that are\u00a0lower\u00a0<\/i>than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.<\/div>\n
For Research Use Only. Not for use in diagnostic procedures.<\/div>\n","protected":false},"excerpt":{"rendered":"

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes\u2122 FluoSpheres\u2122 beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.<\/p>\n","protected":false},"featured_media":3783,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[17],"product_tag":[],"class_list":["post-523","product","type-product","status-publish","has-post-thumbnail","product_cat-laboratory","first","instock","taxable","shipping-taxable","purchasable","product-type-simple"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/bullziye.com\/index.php?rest_route=\/wp\/v2\/product\/523","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bullziye.com\/index.php?rest_route=\/wp\/v2\/product"}],"about":[{"href":"https:\/\/bullziye.com\/index.php?rest_route=\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/bullziye.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=523"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/bullziye.com\/index.php?rest_route=\/wp\/v2\/media\/3783"}],"wp:attachment":[{"href":"https:\/\/bullziye.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=523"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/bullziye.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_brand&post=523"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/bullziye.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_cat&post=523"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/bullziye.com\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_tag&post=523"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}